Earthworm casts restrained the accumulation and phytotoxicity of soil glyphosate to cowpea (Vigna unguiculata (L.) Walp.) plants

Abstract The heavy use of glyphosate during the cultivation of glyphosate-resistant crops, would trigger the so called “pseudo-persistent” glyphosate in soil, thereby threatening agricultural environment, crop production, and human health through food safety. Such that, there is pressing need for the development of strategies for the effective management of glyphosate contamination in soil to promote cleaner agricultural production. In this study, as witnessed via characterizing the bioavailability and phytotoxicity of glyphosate to cowpea plants in soils applied with or without earthworm casts (EWCs), EWCs could significantly facilitate glyphosate desorption from soil, thus enhance its bioaccessibility, nonetheless, sharply decreased rather than increased the accumulation of glyphosate in cowpea plants via reducing the residue pool of glyphosate in the soil. Consequently, in comparison with the glyphosate-alone group, EWCs involvement triggered the increase of chlorophyll content, alleviation of ROS accumulation and lipid peroxidation of membrane , and in turn reduced the activity of a series of stress-tolerance enzymes by means of down-regulating the expression of the corresponding mRNA; ultimately, helped plants to reverse the glyphosate-induced growth suppression. Our findings demonstrated that, EWCs were promising candidate for the cost-effective and easy-to-operate remediation and reuse of glyphosate-contaminated soil, while also being able to improve the quality of the cultivated land and promote crop growth and resistance as a nutrients supplier.