The found of mouse model of adoptive transfer of allogeneic lymphocytes

Objective To establish mouse model of the adoptive transfer of allogeneic lymphocytes. Methods Female BALB/C mice given 5Gy total body irradiation(TBI) were divided into five groups:A, B, C, D, E(n=12), which received male C57BL/6N spleen lymphocytes 5×107, 1×107 and male BALB/C×C57BL/6→F1 spleen lymphocytes 0.5×107, 1×107, 5×107(cells/mouse), respectively. The chimerism of doner lymphocytes was detected by polymerase chain reaction (PCR) after recipient mice survived over 21 days. The survival time and the manifestations of GVHD were observed. Results With 5Gy TBI, the allogeneic lymphocytes could be engraftted successfully. The time of engraftment in MHC all-mismatached group(group B) was shorter than halpoidentical group(group D)(P0.01). The time of engraftment prolonged with the increase of the cell dose infused in C, D, E group,gradually(P0.05). After the engraftment of allogeneic lymphocytes, acute GVHD symptoms were observed. Acute GVHD in MHC all-mismatached group(group A) was more serious than halpoidentical group(group E)(P0.01). Conclusions With 5Gy TBI, the adoptive transferred allogeneic lymphocytes can be engrafted successfully. It is critical to decrease the difference of MHC and adjust the dose of lymphocytes in inducing the stable chimerism of allogeneic lymphocytes without fatal acute GVHD.