Protein analysis of dwarfed transgenic rice plants overexpressing GA2-oxidase gene

Using 2-D electrophoresis, we analyzed proteins from transgenic rice overexpressing gibberellin acid (GA) catabolic enzyme, GA2-oxidase. These results indicate eight specific proteins differentially expressed in the transformed rice stems of T1 generation, but non in case of T2 generation. Proteins isolated from different stages of leaves of T1 generation showed no significant differences, except one-month-old leaf, where five differentially expressed proteins are visible.