Concentration induced properties of silver nanoparticles and their antibacterial study

Abstract Green synthesis of silver nanoparticles (Ag-NPs) using Moringa Oleifera (MO) bloated in flavonoids, tannins, and phenols phytochemical as a capping and reducing agent which reduced the silver ions to Ag-NPs without any external reducing agent for an antibacterial agent is enclave in this work. The effects of the reducing agent in the different concentrations of silver nitrate incubated for 72 h on the structural, morphological and optical properties followed by the antibacterial activity of Ag-NPs were studied. The synthesized Ag-NPs were characterized by scanning electron microscope (SEM), transition electron microscope (TEM), X-ray dispersive spectroscopy (XRD), Uv–Visible spectroscopy and Fourier transform infrared spectroscopy (FT-IR) analysis. The TEM analysis gave a spherical morphology with particle size ranged between 4 – 12 nm. The XRD gave a face-centered cubic phase with the crystalline structure of Ag-NPs. The FTIR shows the functional groups responsible for the reducing agent. The UV-visible confirm the formation of Ag-NPs with surface plasmon resonance (SPR) absorbance peak 450 nm. To our knowledge, the biocidal activities of AgNPs have been studied in details but the biosynthesized formulation of Ag-NPs via MO against Coliform bacterial strain compared with Gentamicin (a strong antibacterial drug) has not been reported in the literature. It is noteworthy that the biocidal activities of Ag-NPs show effective and functional susceptibility to the Ag-NPs against the bacterial strains at lower concentrations compared with Gentamicin. Hence, the obtained Ag-NPs via facile and innocuous synthesis could serve as an auspicious and efficient antibacterial agent against human pathogenic strains due to its non-toxicity, biocompatible, eco-friendly, cost-effectiveness, and facile protocols in therapeutic biomedical fields.