The cholinergic system in retinal transplants in rats.

Abstract Retinas from embryonic day 13 or 14 Sprague-Dawley albino rats were transplanted to the brainstem of newborn rats with unilateral eye enucleation at birth. Two months after the transplantation, the activity and distribution of acetylcholinesterase and choline acetyltransferase were studied using histochemical and immunocytochemical methods respectively. Results obtained showed that the staining patterns of these two cholinergic enzymes in the retinal transplants were essentially the same as those observed in the retinas of normal rats and in the control retinas of the recipient animals. The similarities in the distribution of these two cholinergic enzymes in these retinas suggest that the cholinergic system in the retinal transplants is likely to be functional.