Protein structure determination in living cells by in-cell NMR spectroscopy

This paper determines the first 3D protein structure calculated exclusively on the basis of information obtained in living cells. The model protein used in this 'proof of principle' study is the putative heavy metal-binding protein TTHA1718 from Thermus thermophilus, and it is proposed that the approach could be used to generate accurate high-resolution structures of many other proteins in living environments.