Simultaneous Determination of Malaridine,Sulfadoxine and Pyrimethamine in an Antimalarial Compound Preparation by HPLC

OBJECTIVE To develop a high performance liquid chromatographic(HPLC) method to determine malaridine sulfadoxine and pyrimethamine simultaneously in an antimalarial compound preperation simultaneously.METHODS Buffer(HAc-NaAc,pH 6)-EtOH(70∶30) was used as extraction solvent.The chromatographic analysis was carried out using ZORBAX RX-C_(18) column(4.6 mm×150 mm,5 μm) and mobile phase consisting of buffer[0.022 mol·L~(-1) HCl-0.22% triethylamine,pH 2.3]-ACN(75∶25) at a flow rate of 1 mL·min~(-1).Phenacitine was used as internal standard and all components were quantified with detection wavelength at 266 nm.RESULTS The recoveries of malaridine,sulfadoxine and pyrimethamine were(97.62±1.09)% with RSD=3.68%,(99.77±2.16)% with RSD=2.17% and(98.34±1.94)% with RSD=1.97%(n=3),respectively.The linear range and regression equation of malaridine,sulfadoxine and pyrimethamine were over the range of 20～400 mg·L~(-1) with r=0.999 3,2.05～40.20 mg·L~(-1) with r=0.999 9 and 20.01～800.40 mg·L~(-1) with r=0.999 9.The retention time for the internal standard phenacitine,malaridine,pyrimethamine and sulfadoxine were 10.19,1.27,5.70 and 7.63 min,respectirely.CONCLUSION The method,which is simple,sensitive and precise in the determination and extraction of those three components,can meet the requirements for the quality control of the antimalarial compound preparation.