[Application of positron emission tomography in evaluation of Alzheimer's disease rat model].

Objective To discuss the value of ~(11)C-PIB PET and ~(18)F-FDG micro PET imaging in Alzheimer's disease(AD)rat model. Methods The AD rat model was established by injection of Aβ1-40 into rat's hippocampus. Learning and memory function were estimated by the Morris water maze. Amyloid deposit and neuron loss were observed by Congo red staining and HE staining respectively. ~(11)C-PIB PET and ~(18)F-FDG micro PET scan were performed. Meanwhile the findings of PET imaging were compared with the results of behavior test and histology. Results The uptake of ~(18)F-FDG in hippocampus of AD rat model group was lower than that of control group(P <0.01). The Morris water maze showed that the escape latent period of rat model group was longer than that of control group (P < 0.01).Neuron loss was found in rat model brain. Thus the result of micro-PET imaging was matched with those of the Morris water maze and HE staining. ~(11)C-PIB was specifically bound to 8-amyloid in rat model brain, the uptake of ~(11)C-PIB in AD rat model was higher than that of control ( P < 0.05 ). AB deposit was observed in Congo red staining so that the result of ~(11)C-PIB Micrp-PET imaging was matched with that of B-amyloid deposition. Conclusion ~(11)C-PIB Micrp-PET imaging shows the plaque deposition while ~(18)F-FDG -PET imaging reflects the change of glucose metabolism in hippocampus in AD rat model. And PET scan can be used to verify the successful establishment of AD model rat model. Key words: Alzheimer's disease; Tomography, emission-computed; Amyloid beta-protein