Measurement by SPR of very low dissociation rates: oxidation-mediated loss of biotin-streptavidin affinity.

m), its easeof use, and its compatibility with diverse chemical/biochemicalmethods have rendered the streptavidin–biotin interaction afavorite system in a wide array of biochemical, immunological,and pharmaceutical assays. For example, it is used extensivelyin surface plasmon resonance (SPR) spectroscopy to immobi-lize macromolecules to the surface of biosensor chips.