Production of a Thermostable DNA Polymerase by Site-Specific Cleavage of a Heat-Eluted Affinity Fusion Protein

Torbjörn Gräslund,Joakim Nilsson,A. Michael Lindberg,Mathias Uhlén,Per-Åke Nygren

Production of a Thermostable DNA Polymerase by Site-Specific Cleavage of a Heat-Eluted Affinity Fusion Protein

1997

Torbjörn Gräslund
Joakim Nilsson
A. Michael Lindberg
Mathias Uhlén
Per-Åke Nygren

A novel strategy is described for bacterial expression and affinity purification of a recombinant truncated version of the heat-stable DNA polymerase I fromThermus aquaticus.The DNA polymerase ([De ...

Keywords:

Affinity chromatography
DNA clamp
Polymerase
DNA polymerase
Real-time polymerase chain reaction
Biochemistry
Molecular biology
Tandem affinity purification
Recombinant DNA
Biology
DNA polymerase I

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