Studies on anemia of fish. XII. Hemostatic disorder in common carp induced by exposure to the herbicide molinate.

Hemostatic disorders in common carp induced by exposure to the herbicide molinate were investigated. Fish were exposed to molinate at 0.50ppm for 9 days in Experiment 1, and at 0.32ppm for 13 days in Experiment 2. Blood was collected from all specimens at various intervals, and activated partial thromboplastin times (APTT) and prothrombin times (PT) were determined. A prothrombin correction test was applied to the fish exposed to molinate, and the effect of menadione dimethylpyrimidinol bisulfite (MPB) in preventing the prolongation of clotting time was examined. The hemostatic disorders induced by exposing fish to molinate are summarized as follows: 1) Prolongations were observed in both APTT and PT after 6-7 days. Thereafter, the prolongation of PT became more pronounced than that of APTT. 2) The prolongation of PT was corrected by the addition of stored serum, suggesting that depleted coagulation factors exist in the serum. 3) Menadione was highly effective in preventing the prolongation of clotting times. These facts suggest that the hemostatic disorders resulted from a depletion of vitamin K-dependent factors.