Microalgae chloroplast carrier for efficient cloning and expression and application thereof

The invention discloses a microalgae chloroplast carrier for efficient cloning and expression and application thereof. The microalgae chloroplast carrier provided by the invention comprises a homologous recombination sequence trnI, a homologous recombination sequence trnA, a promoter PrbcL, a terminator TrbcS, an forward XcmI sequence and a reverse XcmI sequence, wherein the forward XcmI sequence and the reverse XcmI sequence are introduced between the promoter PrbcL and the terminator TrbcS so that target gene cloning sites are formed; the homologous recombination sequence trnI is in upstream of the promoter PrbcL; and the homologous recombination sequence trnA is in downstream of the terminator TrbcS. The microalgae chloroplast carrier is characterized in that the promoter and the terminator from the microalgae are utilized to regulate expression of target genes so that the target genes can be efficiently expressed in microalgae chloroplasts.