Influence of Follicle-Stimulating Hormone on the Estimation of Luteinizing Hormone in the Ventral Prostate and Ovarian Ascorbic Acid Depletion Assays

The influence of ovine pituitary follicle-stimulating hormone (FSH) on the measurement of ovine pituitary luteinizing hormone (LH) in the ovarian ascorbic acid depletion (OAAD) and the ventral prostate weight (VPW) assays was examined. It was found that potentiation of ovine luteinizing hormone (NIH-LH-S1) was induced in the OAAD method by ovine follicle-stimulating hormone (NIH-FSH-Sl) when the latter hormone was present in sufficiently high concentration (FSH: LH =30:1 and 100:1). In the VPW assay, the activity of NIH-LH-Sl was not increased by ovine NIH-FSH-Sl at ratios of FSH:LH ranging from 1:1 to 30:1. (Endocrinology 76: 1150, 1965)