HPLC Fingerprint of Chrysanthemum indicum

Objective:To establish HPLC fingerprint of C.indicum to provide the basis for the standards of quality control and identification of the Chinese crude drug.Method: The HPLC method was used on an Agilent Eclipse XDB-C18(4.6 mm×250 mm,5 μm) column with acetonitrile-0.1% acetic acid as mobile phase by gradient elution at the detection wavelength of 290 nm and the column temperature was set at 30 ℃ with the flow rate of 1.0 mL·min-1;the sample injection was 10 μL.Result: HPLC fingerprints of 16 samples of C.indicum were established.16 common peaks were selected as the fingerprint peaks in 11 samples.Among the obtained fingerprints,most of the detected peaks were separated effectively.11 samples had high similarities.Conclusion: The established HPLC fingerprint has desirable accuracy,repeatability and stability,which can be used for one of the quality control of C.indicum.