Induction of NO synthesis in macrophages by Newcastle disease virus is associated with activation of nuclear factor-κB

Newcastle disease virus (NDV) has received much attention recently because of its non-specific immune stimulating potential and Its various anti-tumor activities. Here we describe that NDV induces synthesis of NO and causes an activation of nuclear factor-KB (NF-KB) In murlne macrophages. These reactions were part of an activation process which Included also stimulation of adenosine deaminase and inhibition of 5 -nucleotidase. NDV-mediated NO synthesis and NF-KB activation were blocked by an antloxldant (butylated hydroxyanisole), by an inhibitor of protein tyrosine kinase (genlsteln) and of protein kinase A (H-89), but not by an inhibitor of protein kinase C (staurosporin) . These data suggest that signalling requirements of NF-KB activation and NO production in NDV-treated macrophages are similar.