Effect of Wumei Pill and Its Subdivisions on Notch Signal Pathway in Colonic Epithelial Cells of Ulcerative Colitis in Rats

Objective:To observe the effect of Wumei pill and its subdivisions on Notch signal pathway in colonic epithelial cells of ulcerative colitis(UC) rats,and explore the main therapy and drug of traditional Chinese medicine.Method:The ulcerative colitis model was established by giving 2,4,6-three nitro toluene sulfonate(TNBS) / ethanol enemas.Eighty rats were randomly divided into 8 groups :blank group(physiological saline 10 m L·kg-1),model group(physiological saline 10 m L·kg-1),Wumei pill group(53.2 g·kg-1),cold and heat application group(36.0 g·kg-1),heat group(21.32 g·kg-1),cold group(14.68 g·kg-1),tonic group(6.68 g·kg-1) and astringent group(10.68 g·kg-1).After 8 hours,all the groups were given Wumei pill or its subdivisions once a day.After 10 days,the colon tissue was collected for pathology examination and the Notch-1,Hes-1,Math-1 mRNA expressions of colonic epithelial cells of rats were tested by Real-time quantitative PCR detecting system(Q-PCR) method.Result:Compared with blank group,Notch-1,Hes-1 mRNA expression was significantly enhanced while Math-1 mRNA was decreased in model group rats(P 0.05).Notch-1,Hes-1 mRNA expression was significantly decreased while Math-1 mRNA was enhanced in Wumei pill group and cold and heat application group(P 0.05).Conclusion:The cold and heat application group play an important role in regulating the Notch signal pathway in Wumei pill,and the therapy of cold and heat is the main method for treatment of ulcerative colitis.