Combined Effect of Smoking and Polymorphisms in Tobacco Carcinogen-Metabolizing Enzymes CYP1A1 and GSTM1 on the Head and Neck Cancer Risk in North Indians

Tobacco consumption contributes to the etiology of majority of cancers, and polymorphisms in tobacco-metabolizing enzymes may modulate susceptibility to head and neck cancer (HNC). The aim of this study was to determine whether genetic polymorphisms in genes CYP1A1 and GSTM1, involved in metabolism of major classes of tobacco-derived carcinogens, may predispose to development of HNC in a North Indian population. In this case–control study, 203 HNC patients and 201 control subjects were genotyped for four variants of CYP1A1 using polymerase chain reaction–restriction fragment length polymorphism, and GSTM1 was analyzed for copy number variations by real-time polymerase chain reaction. Haplotype analysis was performed for the CYP1A1 gene using PHASE version 2.1. CYP1A1 CC (T3801C) (odds ratio [OR], 3.49; 95% confidence interval [CI], 1.34–9.05), GSTM1 single copy (OR, 2.63; 95% CI, 1.54–4.51), and null genotypes (OR, 4.37; 95% CI, 2.61–7.29) were found to be significantly associated with an increased risk o...