Acute cytogenetic effects of tyramine, MTCAs, NaCl and soy sauce on rat bone marrow cells in vivo

Abstract The acute cytogenetic effects of tyramine and MTCAs, precursors of the mutagen present in soy souce, were studied with in vivo chromosome aberration test in ra bone marrow cells. The chemicals were administered intraperitoneally. Statistically significant positive results were obtained with tyramine at a dose of 5 mmole/kg (686 mg/kg) body weight and with MTCAs at doses over 0.50 mmole/kg (115 mg/kg) body weight, respectively. Chromosome aberrations (CA) induced by l -proline co-administered with eitherrf tyramine or MTRAs were significantly lower than those induced by each chemical alone. These data suggest that l -proline, after endogenous nitrosation, became nitrosoproline and suppressed CA, and that, as a result of in vivo nitrosation of tyramine and MTCs, they became mutagenic nitroso compounds showing positive results. Statistically significant positive results were obtained by administered of 40 mmole NaCl/kg body weight (2338 mg/kg). The cocarcinogenic role of NaCl with tyramine was suggested because soy sauce contains about 18% NaCl.