Laborchemische Kontrolle der Kallusreifung – eine tierexperimentelle Studie

AIM OF THE STUDY: The purpose of this study was to verify the possibility of monitoring the callus maturation biochemically. METHOD: In an experimental study on sheep, animals with bone fractures with a length 5 cm each were divided into group A (with concomitant damage of the soft tissue) and group B (without soft tissue damage). During callus traction and maturation, biochemical parameters (osteocalcin, AlP, NTx, calcium, phosphate) were measured. Animals of each group (A+B) were euthanised at fixed points in time (according to protocol) and the callus was prepared histologically. RESULTS: Total AlP and NTx values were initially reduced during the callus traction and maturation, but increased significantly at the time of Docking+1 transportation time. At the same time, the histological evaluation showed a definite increase of the bone stabilisation, which approximately resembles the structure of a healthy bone as reference. CONCLUSION: With the combination of the AlP and NTx values already determined in the daily clinical routine the monitoring of callus maturation in animal studies becomes possible.