Population structure and sequence analysis of a Peach latent mosaic viroid isolate from a peach tree showing discoloration along leaf veins.

Peach latent mosaic disease occurred in peach trees is distributed widely in China.The disease shows a wide range of symptoms in the fields and discoloration along leaf veins is easily found.To get insight into the correlation between the symptom and Peach latent mosaic viroid(PLMVd),leaves of a peach tree showing the symptom of discoloration along its veins were collected and subjected to PLMVd RNA extraction,and RT-PCR detection.The target amplified fragment was cloned,and some positive clones were then selected for analyzing population structure of the isolate by single-strand conformation polymorphism(SSCP) analysis.Three predominant clones of the isolate were sequenced and analyzed.This study would provide more information for understanding the correlation between the molecular characterization of PLMVd and the symptom of discoloration along leaf veins.