Preparation of monoclonal antibodies to O-antigen (0–4.5) ofSalmonella typhimurium serogroup B

Sixteen clones secreting murine monoclonal antibodies of various specificities toSalmonella antigens are obtained. One of the clones is highly specific toSalmonella typhimurium (serogroup B) O-antigen in enzyme immunoassay. The resultant antibodies are IgG2a immunoglobulins with a “kappa”-type light chain. The connecting epitope includes abequose-mannose-rhamnose trisaccharide. Antibodies are bound toS. typhimurium whole cells and do not cross-react with representatives of theE. coli family.