THE STUDY OF ANTIBACTERIAL ACTIVITY OF NON-POLAR EXTRACT OF MALVA SILVESTRIS L., USING WELL DIFFUSION AND TUBE DILUTION METHOD

Nowadays, due to the resistance of bacteria to antibiotics and their side effects, much attention is paid to the antibacterial properties of medicinal plants. Malva sylvestis L. plays an important role in traditional medicine and herbal medicine industry. This study deals with antibacterial effects of 50, 100, 200, 400 mg/ml concentration of non-polar extract of Malva sylvestris L. on Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa bacteria by means of well diffusion method. Determination test of MIC (Minimum inhibitory concentration of bacteria growth) and MBC (Minimum bactericidal concentration) was done by tube dilution method. The results indicated that non-polar extract of Malva sylvestris L. inhibits growth of Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa bacteria. The inhibitory effect increases by increasing the extract concentration. MIC and MBC of these bacteria respectively varies from 12.5-50 mg/ml and 12.5-100 mg/ml respectively. This extract can be a good candidate for future in vivo studies for production of new antibacterial drugs.