Abstract PO-002: Epigenetic regulation of metabolic pathways in response of breast cancer cells to stilbenoids

Epigenetics is the study of heritable gene expression changes that are not due to a change in the DNA sequence. Due to the inherent reversibility of epigenetic states, epigenetic modifications, particularly DNA methylation, have attracted a significant amount of attention for the prevention and treatment of cancer. Aberrations in DNA methylation occur during cancer initiation, promotion and progression. Recent evidence suggests that these aberrations may contribute to metabolic disturbances in cancer. Although limited, there are pieces of evidence demonstrating that dietary bioactive compounds, including polyphenols from the stilbenoid subclass, such as resveratrol and pterostilbene, can modulate gene expression by targeting DNA methylation. Our recent investigation into DNA methylation patterns using Illumina 450K BeadChip microarray demonstrates that loci-specific increases and decreases in DNA methylation occur in breast cancer cells in response to resveratrol. We have identified 4,183 differentially methylated CpG sites in MCF10CA1h and 6,347 differentially methylated CpG sites in MCF10CA1a breast cancer cells upon 9-day treatment with 15µM resveratrol as compared with control cells (0.05≤differential methylation≥-0.05, nominal p Citation Format: Barbara Stefanska, Cayla Boycott, Tony Yang. Epigenetic regulation of metabolic pathways in response of breast cancer cells to stilbenoids [abstract]. In: Abstracts: AACR Special Virtual Conference on Epigenetics and Metabolism; October 15-16, 2020; 2020 Oct 15-16. Philadelphia (PA): AACR; Cancer Res 2020;80(23 Suppl):Abstract nr PO-002.