An Extended Genetic Recombination Map for Foot-and-Mouth Disease Virus

Summary The original foot-and-mouth disease virus recombination map (Lake, Priston & Slade, 1975), which included 35 mutagen-induced ts mutants, has been extended both in detail and size by the mapping of a further 33 ts mutants (9 mutagen-induced and 24 spontaneous). The size increase from 0.57% to 3.27% maximum recombination frequency was principally due to the use of a new standardization technique for recombination frequencies but, in addition, the original map distance was increased by approx. 30% due to the mapping of new mutations. As in the original map, there was a marked concentration of mutations near the guanidine (gs) locus, i.e. 83% of the mutants had mutations in the third of the map adjacent to the gs locus.