Persistent Ca2+ Current Contributes to a Prolonged Depolarization in Aplysia Bag Cell Neurons

Neurons may initiate behavior or store information by translating prior activity into a lengthy change in excitability. For example, brief input to the bag cell neurons of Aplysia results in an approximate 30-min afterdischarge that induces reproduction. Similarly, momentary stimulation of cultured bag cells neurons evokes a prolonged depolarization lasting many minutes. Contributing to this is a voltage-independent cation current activated by Ca2+ entering during the stimulus. However, the cation current is relatively short-lived, and we hypothesized that a second, voltage-dependent persistent current sustains the prolonged depolarization. In bag cell neurons, the inward voltage-dependent current is carried by Ca2+; thus we tested for persistent Ca2+ current in primary culture under voltage clamp. The observed current activated between −40 and −50 mV exhibited a very slow decay, presented a similar magnitude regardless of stimulus duration (10–60 s), and, like the rapid Ca2+ current, was enhanced when Ba...