Isolation of Different Dermal Fibroblast Populations from the Skin and the Hair Follicle

: The establishment of primary cells from fresh tissue is a widely used method for investigating human tissue in vitro. The skin harbors different cell populations in the dermis and the hair follicle, which can be isolated for downstream analysis. Here we describe the isolation of four dermal fibroblast populations from human haired skin and their maintenance in culture. The four cell populations for which isolation is described are papillary dermal fibroblast cells, reticular dermal fibroblast cells, hair follicle dermal sheath cells, and hair follicle dermal papilla cells.