LC-MS and 1H-NMR Untargeted Metabolomic Profiling of Probiotic Yogurt Consumption in C56BL6/N Mice

Objectives Probiotic yogurt consumption is associated with gut health, including nutrient production and immune regulation, largely via modulation of the gut microbiome. However, metabolites that mediate host-microbiome interactions are largely unknown. We hypothesize consumption of probiotic yogurt results in distinct metabolic signatures in colon, including decreased levels of branched-chain amino acids, triglycerides, and bile acids relative to probiotic-free yogurt, milk, and water controls. We further hypothesize these differences are driven by increased abundances of Lactobacilli and Bifidobacteria following probiotic yogurt consumption.