Degradation of S. cerevisiae Cohesin with the Auxin-Inducible Degron System
2019
: The cohesin complex is required to establish sister chromatid cohesion and ensure accurate chromosome segregation after DNA replication. Recent data has also revealed a role for cohesion as a major player in DNA repair and gene expression regulation. All subunits of the Cohesin complex are essential and cannot be deleted. Here, we describe a protocol to efficiently deplete cohesin subunits with an auxin-inducible degron (AID) system in S. cerevisiae.
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