Propagation of poliovirus inmicrocarrier cultures of three monkey kidney cell lines

The microcarrier culture system was applied to the propagation of polioviruses in three established monkey kidney cell lines, Vero, LLC-MK 2 and CV-1, and the yields were compared with that obtained in roller bottle and stationary cultures. The yield of the three types of polioviruses in the various cell lines and culture systems were similar. However, the use of the microcarrier culture system for anchorage-dependent cells together with the use of continuously propagating cell lines offer great advantage for large-scale cultivation of poliovirus for the preparation of killed poliovirus vaccines.