Fructose diphosphatase from rabbit liver: V. Subunit structure of the purified enzyme
1966
Abstract Fructose 1,6-diphosphatase purified from rabbit liver has a molecular weight of about 127,000. The enzyme is readily dissociated into two subunits by exposure to pH 2.0. Partial reconstitution of the native protein structure and recovery of enzyme activity is obtained by dilution of the dissociated enzyme at neutral pH. Preliminary analyses of the carboxy- and amino-terminal amino acids confirms the existence of two polypeptide chains and suggests that these are not identical.
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