Alginate-lyase Guided Silver Nanocomposites for Eradicating Pseudomonas Aeruginosa from lung

Pseudomonas aeruginosa (P. aeruginosa) infections lead to a high mortality rate for cystic fibrosis or immunocompromised patients. The alginate of the biofilm was believed to be the key factor disabling immune therapy and antibiotic treatments. A silver nanocomposite consisting of silver nanoparticles and a mesoporous organosilica layer was created to deliver two pharmaceutical compounds (alginate-lyase and ceftazidime) to degrade the alginate and eradicate P. aeruginosa from the lungs. The introduction of thioether-bridged mesoporous organosilica into the nanocomposites greatly benefited the conjunction of foreign functional molecules such as alginate-lyase and increased their hemocompatibility and drug-loading capacity. Silver nanocomposites with a uniform diameter (~ 39 nm) exhibited a high dispersity, good biocompatibility, and high ceftazidime loading capacity (380.96 mg/g). Notably, the silver nanocomposites displayed a low-pH dependent drug-release and degradation profiles (pH 6.4), guaranteeing th...