A study on the esterase isoenzyme of the variants derived from barley DNA introduction into common wheat

Five stable plant-lines from resistance variants in D_(2) generation derived from introducing DNA of two-rowed barley (H.vulgare ssp. distichon Hsu)with resistance to powdery mildew into susceptable common wheat ‘Hua 76’via the pollen-tube pathway were analysed with esterase isoenzyme(EST). The results of EST detection showed that the lines increased one band which was present in the donor but was absent in the receptor, named as transgene enzyme band. Thus it is confirmed that barley genes (or DNA segments)were integrated into or recombined with receptor^s wheat genome and expressed. The effects of exogenous DNA introduced into recipient on variation of the character and change of the isoenzyme activity in the progeny were also discussed.