Detection of mobile genetic elements in multidrug-resistant Klebsiella pneumoniae.

OBJECTIVE To investigate the distribution of the drug-resistant genetic carrier-mobile genetic elements in multidrug-resistant Klebsiella pneumoniae.METHODS Four kinds of mobile genetic elements,such as conjugal plasmid genetic marks(traA and trbC),cut-in sequence genetic marks(IS1133 and ISEcp1),transposon genetic marks(merA,tnpU and tnp513) and integron genetic marks(intⅠ1,intⅠ2 and intⅠ3) in 20 cluster MDR-KPN strains were assayed by polymerase chain reaction(PCR).RESULTS Among 20 strains of MDR-KPN,the positive rates of trbC,ISEcp1,merA,tnpU,tnp513,intⅠ1 were 55.0%,65.0%,25.0%,20.0%,50.0% and 65.0%,respectively.and the other 4 genes were negative.At least one mobile genetic element was found in each MDR-KPN strain.It was first reported that ISEcp1,merA,tnpU and trbC were found positive in MDR-KPN in china.CONCLUSIONS Mobile genetic elements can be mostly found positive in MDR-KPN.trbC,ISEcp1,merA,tnpU,tnp513,and intⅠ1 may be the main mobile genetic elements which induce multi-resistant mechanism of MDR-KPN in our hospital.