Effects of different factors on direct induction of microtubers from explants in Pinellia ternate

Objective: To study the effects of different factors on direct induction of microtubers. These factors included plant growth substances, casein hydrolysate (CH), active carbon (AC), polyethylene glycol (PEG 4000), sucrose and glucose. Method: Using the orthogonal design method. Result and Conclusion: The optimal media to directly induce microtubers from leaves were MS+MET (0.5 mg·L~(-1)+6)-BA 0.5 mg·L~(-1)+sucrose 3% and MS+6-BA 0.5 mg·L~(-1)+IAA 0.5 mg·L~(-1)+sucrose 3%. Optimal media to directly induce microtubers from tubers were MS+6-BA 1.0 mg·L~(-1)+PEG 5%+sucrose 5% and MS+CH 500 mg·L~(-1)+ MET 0.5 mg·L~(-1)+6-BA 0.5 mg·L~(-1)+AC 0.5%+sucrose 5%. Media suitable for plantlet growth were MS+6-BA 0.5 mg·L~(-1)+IAA 0.5 mg·L~(-1)+(AC 0.5%+)sucrose 5% and MS+MET 0.5 mg·L~(-1)+6-BA 0.5 mg·L~(-1)+sucrose 3%.