Unfolding of Population Structure in Dehong Buffalo Using Microsatellite DNA Markers
2007
Dehong buffalo are an indigenous buffalo breed in Yunnan province,
China. A total of 81 unrelated samples from Dehong buffalo breeds were
genotyped using 15 microsatellite DNA markers from 14 chromosomes to
estimate genetic diversity, genetic differentiation and gene flow
within populations. A total of 62 alleles were detected across the 15
loci assayed. All the loci were polymorphic and the number of alleles
ranged from two to six, giving a mean number of 4.13 alleles per locus.
The expected heterozygosity and polymorphism information content of 15
microsatellite loci were 0.6520±0.1526 and 0.5863±0.1789,
respectively. Coefficients of genetic differentiation ranged from 0 to
0.0919, with the average of 0.0202. Gene flow at each locus was large,
with a mean Nm of 12.1502. The results indicated that the genetic
diversity was rich among Dehong buffalo and the genetic differentiation
in its sub-populations was relatively low, with larger gene flow and
less inbreeding.
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