Absence de maladie vésiculeuse du porc en Suisse : enquête séro- épidémiologique par ELISA (1985-1987): -EN- Absence of swine vesicular disease from Switzerland: results of a sero-epidemiological survey using ELISA (1985-1987) -FR- -ES-

Summary: Since there has been no evidence of swine vesicular disease (SVD) in Switzerland since 1973, and because the disease can circulate in an inapparent form, a sero-epidemiological survey was undertaken. For this purpose, an ELISA was developed, using a cytoplasmic extract from infected and uninfected cell cultures. This extract was later analysed by PAGE and immunoblotting, and was shown to contain the structural viral proteins VP0, VP1, VP2, VP3 and VP4. Having shown a satisfactory correlation between ELISA and the serum neutralisation test, the former was employed to test 9,529 serum samples from breeding pigs. Of these, 9,358 (98.2%) were negative, 8 (0.09%) were positive and the remaining 163 (1.71%) could not be interpreted. Positive samples and those impossible to interpret were retested by the serum neutralisation test against SVD virus and porcine enterovirus serotypes 1, 2, 3, 6 and 8. These sera neutralised the other enterovirus serotypes to a greater extent than the SVD virus. Thus, it would appear that the positive reactions in the anti-SVD virus ELISA were due to antibodies against other enterovirus serotypes.