Establishment of real-time fluorescent PCR detection method of invA gene in salmonella

A pair of specific primers was designed according to invA gene of salmonella. The SYBR Green real-time PCR method for detecting salmonella was established. The specificity,sensitivity and repeatability of this method was analyzed. The result showed that this method were good specificity and repeatability,the detection of salmonella minimum copy number was 1×10CFU/mL. Therefore,the study established the salmonella specific, sensitive and rapid real-time fluorescence PCR detection method.