The feasibility of negative pressure suction enteroscopy for small intestine examination

Objective To evaluate the feasibility of negative pressure suction enteroscopy for intesti- nal examination. Methods The negative pressure suction enteroseopy was first applied to examine 20 de- tached small intestines from pigs to explore a suitable work pressure and to detect possible intestinal dama- ges. The procedure was then applied in living pigs to evaluate the feasibility and to observe possible adverse reactions in a 2-week follow-up. Results The absolute working pressure of the negative pressure suction en- teroseopy should be higher than 0. 06 Mpa. The procedures were successfully completed in all 20 pig small in- testines in 40 ± 20 min. There was no tissue damage in the intestinal wall according to histological findings. Local glide occurred in living pig examination at 0.06 Mpa, and it disappeared when the absolute value was higher than 1.00 Mpa. The procedure was completed in 20 living pigs in 60± 20 min. Histological findings showed complete tissue structure, clear villi and complete submucous and muscular tissue. All pigs could take food at recovery from anaesthesia. 18 had bowel movement on the 2nd day after the operation, and the 2 others recovered on the 3rd day. No abdominal distension, hematochezia, or constipation was observed in 2-week fol- low-up. Food intake, activity, sleep, urination and defecation were all normal. Conclusion The negative pressure suction enteroscopy is effective and safe for small intestine examination, and is of good prospect. Key words: Animal experimentation;  Endoscopy ;  Intestine, small;  Negative pressure suction small intestine enteroscopy