Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis

gene to chromosome 1 and show that alternativepolyadenylationsignalsprovideamechanismforgeneratingtwodistinct isoforms. Sequencing of products generated by reversetranscriptase PCR, immunoblotting of cell extracts and trans-fection of antisense oligonucleotides together demonstrate thathuman Nek2 is expressed as two splice variants. These isoforms,designated Nek2A and Nek2B, are detected in primary bloodlymphocytes as well as adult transformed cells. Nek2A andNek2B, which can form homo- and hetero-dimers, both localizetothecentrosome, althoughonly Nek2Acaninduce centrosome