Method for preparing high-sensitivity PK15 cell line L8 of porcine circovirus type 2

The invention discloses a method for preparing a high-sensitivity PK15 cell line L8 of porcine circovirus type 2. The method comprises the following steps: (1) resuscitating a PK15 cell and then cultivating in a cell bottle to grow into a cell monolayer, digesting by using pancreatin of which the mass fraction is 0.25%, and then adding DMEM (Dulbecco Modified Eagle Medium) growth liquid to blow and beat, wherein the DMEM growth liquid contains fetal calf serum of which the mass fraction is 10% and double antibodies of which the mass fraction is 1%, dispersing the cell into single cells, and carrying out cell cloning by using a series of dilution methods; and (2) screening out a single cell line with high sensitivity on PCV2 through indirect immunofluorescence. A cell line with higher infection rate on PCV2 is obtained by adopting a more convenient and efficient single cell clone technology, named as L8. It is found that the proliferation titer of the PCV2 in the cell can be stably improved by the cell line by detection of the stability and the purity of the cell line.