Engineered metal-binding proteins: purification to protein folding
1991
Proteins can make use of metal ions to bind substrates,
to maintain structure, to effect catalysis, and for
allosteric control and regulation. In order to hold a particular
metal ion with high affinity and specificity, proteins form multidentate
binding pockets designed to fulfill both the chemical and
geometric bonding requirements of that metal. Metal recognition
can be engineered into proteins for applications such as protein
purification.
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