Selective detection of13C-containing metabolites by1H double-resonance BLISS sequence

Recently there has been a great deal of interest in double-resonance methods capable to enhance the detection sensitivity of less sensitive nuclei such as13C in liquid system, in order to study brain metabolism noninvasively. The main disadvantage of the existing1H-observed/13C-editing double resonance techniques, based on intermolecular short-rangeJ-coupling interaction, is the nontotal proton solvent elimination. The indirect13C-editing and spatial localisation in 10 mM solution of enriched glucose in water, was obtained in our laboratory by means of the BLISS (bilinear and linear interaction spin-selection and solvent suppression) sequence with total solvent elimination. The sensitivity gain and the water suppression are here theoretically calculated and experimentally verified.