Transcriptional analysis of intracytoplasmically stained, FACS-purified cells by high-throughput, quantitative nuclease protection
2009
Cells that have been purified by FACS using intracellular markers are not amenable to gene expression analysis by conventional methods. Pechhold et al. solve this problem with the quantitative nuclease protection assay and apply the approach to study subsets of islet cells.
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