Determination of 3 Kinds of Components in Compound Xueyaan Sustained Release Tablets by HPLC

OBJECTIVE: To establish HPLC method for the determination of the contents of atenolol, hydrochlorothiazide and nifedipine in xueyaan sustained release tablets. METHODS: The determination was performed on ZORBAX SB C18 column with column temperature at 35℃ . The mobile phase consisted of 0. 01mol· L- 1 sodium dihydrogen phosphate solution- methanol- acetonitrile ( 6∶ 3∶ 1) . The UV detection wavelength was set at 272nm and the sample size was 20μ L. RESULTS: The liner ranges of atenolol, hydrochlorothiazide and nifedipine were 10～ 250μ g· mL- 1 ( r=0. 999 4) , 6～ 150μ g· mL- 1( r=0. 999 6) , and 5～ 125μ g· mL- 1( r=0. 999 2) respectively. The average recovery rates of the three constituents were ( 98. 51± 0. 98) % ～ ( 99. 12± 0. 98) % , ( 98. 87± 1. 10) % ～ ( 99. 69± 1. 43) % , and( 98. 80± 1. 27) % ～ ( 99. 38± 1. 13) % , respectively, with RSD at 1. 00% , 1. 17% , and 1. 25% , respectively. CONCLUSION: The established method is simple, sensitive and reproducible, and suitable for the quality control of xueyaan sustained release tablets.