Breeding Fusarium Wilt and Verticillium Wilt of Cotton-Resistant Strains With Transgene Technique

Using pollen tube pathway methed the bivalent plant propess carrier for bean chitin enzyme gene tobacco β 1,3 glucanose gene were transferred to the strains of cotton in local,550,1304,822 and shiyuan 321.Pass through kanamycin check, PCR Southern blot test, screen for the character of fusarium wilt and verticillum wilt of cotton resistant, the transgenic plant of cotton that had good resistant and stable heredity were obtained.After selection for 10 generations the new strains of cotton of fusarium wilt and verticillum wilt of cotton resistant or endurance were bred.