Research on the Signals of Cotton in Response to UV-B Radiation

Phenotype and hydrogen peroxide and nitric oxide were studied in three kinds of cotton under ultraviolet-B(UV-B) radiation with or without SNP and ABA treatment.The results indicated that leaves of cotton seedlings showed obvious rust,and leaves in vitro dehydrate apparently suffering UV-B stress.Pretreatment with sudium nitroprusside(SNP) and abscisic acid(ABA) lightened rust and wilt symptom of the leaves due to UV-B radiation.Both hydrogen peroxide(H2O2) dyed with 3,3-diaminobenzidine(DAB) and nitric oxide(NO) labelled by 4,5-diaminofluorescein diacetate(DAF-2DA) were increased after UV-B radiation than untreated control.And the release of NO alleviated the damage from UV-B radiation by reducing the accumulation of H2O2.In additon,pretreatment with ABA significantly increased the tolerance of cotton,which is bound up with the production of H2O2 and NO.Therefore,UV-B radiation caused damage of cotton during the growth and development,and all of H2O2,NO and ABA are members of the signal transduction during the interaction between cotton and UV-B stress.