Flow-cytometry-based kit for high-flux screening of ethanol-producing fungi and application thereof

The invention discloses a flow-cytometry-based kit for high-flux screening of ethanol-producing fungi and application thereof. The kit comprises a box body, reagents, a cell culture plate, a centrifuge tube and a copper screen. The reagents are as follows: 1000 mL of reagent A composed of 6.15g of citric acid and 17.544g of sodium citrate, reagent B composed of 0.3mg of propidium iodide (PI) and 0.3mg of DCFH-DA, 50 mL of reagent C composed of 2g of glucose, 50 mL of reagent D composed of 10g of glucose, 50 mL of reagent E (pH value is 5.5) composed of 0.5g of yeast powder, 0.5g of tryptone, 0.05g of ammonium monoacid phosphate and 0.025g of magnesium sulfate, and 50 mL of reagent F (pH value is 5.5) composed of 0.05g of yeast powder, 0.05g of tryptone, 0.05g of ammonium monoacid phosphate and 0.025g of magnesium sulfate.