Monoclonal Antibodies Against Membrane Glycolipids of an Anti-Streptococcal Group a b-Cell Hybridoma

Abstract A B-cell hybridoma producing monoclonal antibody specific for Streptococcal Group A polysaccharide was used as a source of murine lymphocyte membrane glycolipids. Mouse monoclonal antibodies were raised against purified gangliosides and neutral glycosphingolipids and were detected using an ELISA assay. We report the preliminary characterization of the neutral glycosphingolipid antigens recognized by two hybridomas.