MOESM7 of Defective angiogenesis in CXCL12 mutant mice impairs skeletal muscle regeneration

Additional file 7: Figure S6. (Related to Figure 5). Quantitative histological and ultrastructure parameters of the vascular network of muscle one month post FI in WT and KI mice. (A) Quantification of the sprouting vessels number one month post FI in the muscle of WT (Flk1GFP/+) and KI (CXCL12Gagtm/Gagtm :: Flk1GFP/+) mice (n=5). (B and E) Scanning Electron Microscopy (SEM) sample were selected according to Sirius Red staining. Scale bar represent 200 μm. (C and D) A set of representative images of a satellite cell (SC); a myofiber (MF); an endothelial cell (EC); a pericyte (PC) from WT (C57Bl6) muscle. Scale bar represent 1 μm. (F-G) Representative images of a SC, a MF a EC and a PC from two different zones selected on Sirius Red section in KI (CXCL12Gagtm/Gagtm) muscles: (F) in a fully regenerated area and (G) in the non-regenerated zone. Scale bar represent 1 μm. (H) Number of pericytes identified around vessels in the non-regenerated zone of KI muscle compared to WT muscle one month post FI. Ten fileds including at least one vessel were randomly counted on each TA section for each condition (n=3 animals per condition). Data are given as the mean ± SEM. p < 0.05; ** p < 0.01.