Effects of feeding method on production of glutathione by Saccharomyces cerevisiae

Several feeding fed-batch culture methods for the production of glutathione (GSH) by Saccharomyces cerevisiae were compared. The results showed that the feeding could obviously promote the growth of S.cerevisiae and the synthesis of glutathione, and different feeding methods had different influences on the biomass and GSH in the broth. The highest biomass could obtain by exponential feeding, but the concentration of GSH was lower; higher biomass and higher GSH could be obtained by pH-stat fed-batch culture, the total concentration of GSH (977.8mg/L) was highest.